Purification of TEM-1 beta-lactamase by immunoaffinity chromatography.
نویسنده
چکیده
A monoclonal antibody prepared against TEM-1 beta-lactamase was found to compete with penicillins and cephalosporins for binding to the enzyme. The purified antibody preparation was linked to Sepharose 4B and used for immunoaffinity-chromatography purification of TEM-1 beta-lactamase. Elution with either benzylpenicillin or cloxacillin yielded a highly purified, concentrated and active enzyme preparation.
منابع مشابه
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 263 1 شماره
صفحات -
تاریخ انتشار 1989